In the cycle sequencing method, how is the sequence of the DNA determined?
A)from autoradiograms
B)from the order of fluorescent colors in the capillary gel
C)from the overall color of the gel
D)from the amount of heavy nitrogen in each band on the gel
E)from the absorbance read in the spectrophotometer

Question

Quizplus · Accepted Answer

In the cycle sequencing method, fluorescently-labeled nucleotides are incorporated into the growing DNA strand as it is replicated in cycles. The order of the fluorescent colors in the capillary gel corresponds to the sequence of the DNA being sequenced, and can be read by a computer.

In the Cycle Sequencing Method, How Is the Sequence of the DNA