If the template in the cycle sequencing method is a PCR product, what is generally used as a primer?
A)both PCR primers
B)randomly generated primers
C)only one of the PCR primers
D)any primer except the PCR primers
E)poly(A)oligonucleotides

Question

Quizplus · Accepted Answer

Generally, only one of the PCR primers is used as the sequencing primer, as it will anneal to the end of the template strand and allow for sequencing in the desired direction. Using both PCR primers could result in sequencing of the opposite strand or amplification artifacts. Randomly generated primers, poly(A)oligonucleotides, or any primer except the PCR primers would not be specific to the desired template strand and could result in incorrect or incomplete sequencing.

If the Template in the Cycle Sequencing Method Is a PCR