In the PCR quantification procedure involving molecular beacons, what causes the fluorochrome to fluoresce?
A)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity degrades the fluorochrome.
B)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity separates the fluorochrome from the quencher.
C)The degradation of the reporter oligonucleotide by DNA polymerase exonuclease activity degrades the fluorochrome.
D)The degradation of the reporter oligonucleotide by DNA polymerase endonuclease activity separates the fluorochrome from the quencher.
E)DNA polymerase combines the fluorochrome and the quencher.

Question

Quizplus · Accepted Answer

In molecular beacons used for PCR quantification, fluorescence occurs when the DNA polymerase's 5' to 3' exonuclease activity degrades the reporter oligonucleotide, separating the fluorochrome (the fluorescent molecule) from the quencher molecule. This separation allows the fluorochrome to fluoresce, which can then be detected and quantified.

In the PCR Quantification Procedure Involving Molecular Beacons, What Causes