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Biochemistry Study Set 8

Chemistry

Quiz 5 :

Methods in Protein Biochemistry

Quiz 5 :

Methods in Protein Biochemistry

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The following peptides are separated using an anion exchange resin in ion-exchange chromatography. Which peptide is eluted first? img
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D

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Which peptide would migrate the slowest in an SDS-PAGE gel? img
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C

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Which percentage of polyacrylamide would give the best separation for large proteins?
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A

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Why is the process of purifying proteins from cells considered challenging?
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After centrifugation, there is a 10% decrease in activity and a 75% decrease in total protein. What is purification of the target protein?
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When preparing to isolate proteins from plant cells, the first step in preparing the cell homogenate would be
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Calculate the purification of the target protein when there is a 30% decrease in activity and a 55% decrease in total protein after centrifugation.
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Which peptide would migrate the fastest in a SDS-PAGE gel? img
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The figure below shows three proteins that are separated using gel filtration chromatography. Which protein is the largest? img
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The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the cytosol fraction? img
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Which separation technique exploits the solubility differences of proteins?
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After centrifugation, the purity of the protein is determined by
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Calculate the specific activity when 500 mg of protein has an activity of 18,000 units.
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Which percentage of polyacrylamide would give the best separation for small proteins?
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The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the membrane fraction? img
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Which type of chromatography separates proteins using specific binding properties?
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Which of the following peptides would be eluted last when separated using gel filtration chromatography? img
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The figure below shows how differential centrifugation can be used to isolate four subcellular fractions. Which fraction corresponds to the mitochondrial fraction? img
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Biochemical assays are important in the process of isolating proteins because they
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After using the salting out method to isolate proteins, how is ammonium sulfate removed from the solution?
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