Since dideoxy sequencing is based on the chain termination, why are normal deoxynucleotides also included in the reaction?
A) to produce a range of DNA synthesis products that terminate at every occurrence of a particular base
B) to provide a substrate for DNA polymerase
C) to create DNA synthesis products long enough to allow running a gel
D) to enhance the chain termination ability of the deoxynucleotides

Question

Quizplus · Accepted Answer

Including normal deoxynucleotides allows the DNA polymerase to synthesize DNA strands of varying lengths, terminating at every possible position of the target base, which is crucial for determining the sequence.

Since Dideoxy Sequencing Is Based on the Chain Termination, Why