Which of the following is in the correct order for one cycle of PCR?
A) Anneal primers; denature DNA; extend primers.
B) Add fresh enzyme; denature DNA; anneal primers; add dNTPs; extend primers.
C) Denature DNA; anneal primers; extend primers.
D) Extend primers; anneal primers; denature DNA.
E) Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.

Question

Quizplus · Accepted Answer

The correct order for one cycle of PCR is denature DNA; anneal primers; extend primers. This allows the primers to attach to their complementary sequences on the DNA template and the polymerase enzyme to extend the primers, creating a new strand of DNA. Option A and D have the correct steps but in the wrong order. Option B and E involve additional steps that are not required for one cycle of PCR.

Which of the Following Is in the Correct Order for One