What is a significant problem with regard to making cDNA libraries to analyze overall gene function in an eukaryotic organism?
A) A cDNA library will only contain genes that are actively transcribed in the organism.
B) Reverse transcriptase cannot be used to make cDNA libraries.
C) cDNA cannot be amplified by PCR.
D) cDNA libraries do not contain intron sequences.
E) cDNA libraries may contain several alternatively spliced forms of the same gene.

Question

Quizplus · Accepted Answer

A significant problem with making cDNA libraries to analyze overall gene function in an eukaryotic organism is that a cDNA library will only contain genes that are actively transcribed in the organism. This means that genes that are normally expressed at low levels or are only expressed in certain tissues or under specific conditions may not be present in the library, leading to a biased representation of the organism's transcriptome.

What Is a Significant Problem with Regard to Making CDNA