A method of determining if proteins are interacting in vivo that makes use of a transcription factor involved in regulating galactose metabolism genes is:
A) a DNA microarray.
B) yeast two-hybrid analysis.
C) tandem affinity purification.
D) immunofluorescence.
E) quantitiative PCR.

Question

Quizplus · Accepted Answer

Yeast two-hybrid analysis is a method that detects protein-protein interactions in vivo by fusing one protein to the DNA-binding domain of a transcription factor and another protein to the activation domain of the same transcription factor. If the two proteins interact, the transcription factor is reconstituted, leading to activation of reporter genes such as those involved in galactose metabolism. This enables easy detection of interacting proteins.

A Method of Determining If Proteins Are Interacting in Vivo