Which of the following is true regarding gel-filtration chromatography and PAGE?
A) In both, small proteins move most rapidly.
B) In both, large proteins move most rapidly.
C) In PAGE, large proteins move most rapidly but in gel filtration, small proteins move most rapidly.
D) In gel filtration, large proteins move most rapidly, but in PAGE, small proteins move most rapidly.
E) None of the above.

Question

Quizplus · Accepted Answer

In gel filtration chromatography, larger molecules elute first because they are not trapped in the porous matrix of the gel, allowing them to move more rapidly through the column. In PAGE (Polyacrylamide Gel Electrophoresis), smaller proteins move more rapidly through the gel matrix because their smaller size allows them to navigate through the pores of the gel more easily than larger proteins.

Which of the Following Is True Regarding Gel-Filtration Chromatography and PAGE