Two-dimensional electrophoresis is a combination of which two techniques?
A) isoelectric focusing and affinity chromatography
B) ion-exchange chromatography and SDS-PAGE
C) affinity chromatography and SDS-PAGE
D) isoelectric focusing and SDS-PAGE
E) isoelectric focusing and ion-exchange chromatography

Question

Quizplus · Accepted Answer

Two-dimensional electrophoresis involves the separation of proteins based on their isoelectric point (isoelectric focusing) in the first dimension, followed by separation based on their molecular weight (SDS-PAGE) in the second dimension. Therefore, the correct answer is option D.

Two-Dimensional Electrophoresis Is a Combination of Which Two Techniques