Which of the following statements relative to the maximum velocity (Vmax) of an enzyme catalyzed reaction (assayed under conditions of excess substrate) is correct?
A) Vmax is equal to twice the Km.
B) Vmax is directly proportional to the concentration of the substrate.
C) Vmax is reached when the concentration of the enzyme-substrate complex is equal to the original concentration of the enzyme.
D) Vmax is reached if an enzyme concentration is used that is at least ten times higher than the Km.
E) Vmax is independent of the enzyme concentration.

Question

Quizplus · Accepted Answer

Vmax is reached when all the enzyme molecules are bound to substrate molecules, meaning the concentration of the enzyme-substrate complex is equal to the original concentration of the enzyme. This condition reflects that all active sites of the enzyme are occupied, and the reaction rate cannot increase further by adding more substrate.

Which of the Following Statements Relative to the Maximum Velocity