In general, PCR primers need to have which of the following characteristics?
A) Each primer needs to base-pair with a site on one of the two DNA strands.
B) The primers need to base-pair with each other.
C) Each primer needs to base-pair with two sites on each of the two DNA strands.
D) The two primers need to base-pair with sites that are repetitive in the genome.

Question

Quizplus · Accepted Answer

PCR primers need to base-pair with a specific site on one of the two DNA strands to initiate amplification. The primers do not necessarily need to base-pair with each other or require repetitive sites in the genome, and each primer only needs to base-pair with one site on one of the strands. Therefore, options B, C, and D are incorrect.

In General, PCR Primers Need to Have Which of the Following