Enzymes necessary for the construction of a recombinant molecule of DNA are
A) restriction endonucleases and DNA ligase.
B) restriction endonucleases and Pol I.
C) Pol I and DNA ligase.
D) recombinase and DNA gyrase.

Question

Quizplus · Accepted Answer

Restriction endonucleases are necessary to cut the DNA at specific sites and generate sticky ends that can be easily joined together. DNA ligase is then necessary to seal the gaps between the two DNA fragments and create a stable recombinant molecule. Pol I is not typically used in recombinant DNA technology and recombinase and DNA gyrase are not necessary for the construction of a recombinant DNA molecule.

Enzymes Necessary for the Construction of a Recombinant Molecule of DNA