If a cell-free protein-synthesizing system from bacteria were supplied with aminoacyl-tRNAs rather than free amino acids, which of the following would NOT be required for peptide bond synthesis?
A) ATP
B) mRNA
C) ribosomes
D) peptidyl transferase
E) GTP

Question

Quizplus · Accepted Answer

ATP is not required because the amino acids are already attached to their respective tRNAs, a process that normally consumes ATP. In this scenario, since aminoacyl-tRNAs are supplied, the energy-consuming step of amino acid activation (which requires ATP) is bypassed.

If a Cell-Free Protein-Synthesizing System from Bacteria Were Supplied with Aminoacyl-TRNAs