Splice site specificity is determined by:
A) conserved sequences within the intron.
B) conserved sequences within the exons.
C) U4 and U6 snRNAs.
D) RNAase III.
E) RNAase P.

Question

Quizplus · Accepted Answer

Splice site specificity is primarily determined by conserved sequences within the intron, specifically the regions upstream and downstream of the intron-exon boundaries. These regions contain the 5' splice site (GU) and the 3' splice site (AG) consensus sequences, as well as other regulatory sequences that help to define the precise location of splicing. Exons also contain important sequences that help to regulate splicing, but they are not as critical for determining splice site specificity as the intronic regions. U4 and U6 snRNAs are involved in the spliceosome complex that mediates splicing, while RNAase III and RNAase P are involved in RNA processing and maturation, but they do not directly determine splice site specificity.

Splice Site Specificity Is Determined By