To obtain information about the precise shape of a globular protein an investigator should perform
A) affinity chromatography.
B) fluorescence spectroscopy.
C) ion exchange chromatography.
D) polyacrylamide electrophoresis in the presence of sodium dodecylsulfate.
E) x_ray diffraction.

Question

Quizplus · Accepted Answer

X-ray diffraction would provide information about the precise shape of a globular protein. This technique involves shooting X-rays at a crystal of the protein and analyzing the pattern of diffraction that results. This pattern can be used to determine the positions of the atoms in the protein, allowing for a precise determination of its shape. The other techniques listed could provide useful information about the protein, but would not necessarily provide information about its precise shape.

To Obtain Information About the Precise Shape of a Globular