A vector designed for the expression of a eukaryotic transgene in E.coli requires a
A) eukaryotic promoter and bacterial transcription termination sequences.
B) eukaryotic promoter and bacterial ribosome binding sequences.
C) bacterial promoter and bacterial transcription termination sequences.
D) bacterial promoter and bacterial ribosome binding sequences.
E) bacterial promoter, bacterial transcription termination sequences, and bacterial ribosome binding sequences.

Question

Quizplus · Accepted Answer

For the expression of a eukaryotic transgene in E. coli, a bacterial promoter is needed for initiation of transcription by the bacterial RNA polymerase, bacterial transcription termination sequences are required to properly end transcription, and bacterial ribosome binding sequences (such as the Shine-Dalgarno sequence) are necessary for the initiation of translation by bacterial ribosomes.

A Vector Designed for the Expression of a Eukaryotic Transgene