You are interested in determining which genes are being expressed in a muscle cell at a particular time in the development of a chicken.For this, you will most likely use
A) a cDNA library.
B) a genomic library.
C) CRISPR technology.
D) antisense RNA.
E) interference RNA.

Question

Quizplus · Accepted Answer

A cDNA library would be the best choice because it represents the expressed genes in a particular cell or tissue at a given time. By using reverse transcription to convert mRNA to cDNA and cloning the resulting cDNAs into a library, researchers can identify which genes are being transcribed and are therefore active in the muscle cell at the developmental stage of interest. Genomic libraries, CRISPR technology, antisense RNA, and interference RNA are not as useful for identifying which specific genes are actively expressed in a particular cell type.

You Are Interested in Determining Which Genes Are Being Expressed