Biologists engaged in recombinant DNA technology often will cut the vector with two different restriction enzymes that have nearby restriction sites.This is done to
A) increase the size of the vector.
B) increase the probability that the vector will recircularize without the transgene.
C) decrease the probability that the vector will recircularize without the transgene.
D) activate the transgene.
E) inactivate the transgene.

Question

Quizplus · Accepted Answer

Cutting the vector with two different restriction enzymes that have nearby restriction sites decreases the probability that the vector will recircularize without the transgene. This is because the two cuts create two separate fragments that must be joined together in the correct orientation to recircularize the vector. This reduces the likelihood of the vector recircularizing without the transgene inserted between the two fragments.

Biologists Engaged in Recombinant DNA Technology Often Will Cut the Vector