Which of the following statements about PCR is/are FALSE?
A) PCR reaction contains DNA template,primers,deoxyribonucleotide triphosphates,and heat-resistant DNA polymerase.
B) Each PCR cycle includes three steps: denaturation,annealing,and extension.
C) After each PCR cycle,amount of DNA increases in a linear manner.
D) One limitation in PCR is that it requires prior knowledge of the sequence or at least part of the sequence to be amplified.
E) PCR is a very sensitive technique and can amplify target sequences present in extremely low copy numbers.
Correct Answer:
Verified
Q2: The polymerase chain reaction requires ssDNA primers
Q3: Which of the following is NOT true
Q4: Which of the following sequences includes a
Q5: Gel electrophoresis can be used to separate
Q6: A radioactive probe is generated using the
Q7: In the Sanger sequencing method,the use of
Q8: Recombinant DNA techniques typically require the action
Q9: A plasmid vector has a gene for
Q10: The cDNA for a eukaryotic gene B
Q11: Assume that a cosmid will carry inserts
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