In lab, Tom was given a mixed culture.His objective is to isolate single colonies.What should be used to accomplish this goal?
A)sterile swab, loop dilution, TSB
B)hockey stick, spread plate technique, and 4 degree Celsius incubation temperature
C)loop dilution, TSA, hockey stick, 37 degree Celsius incubator
D)inoculating loop, incinerator, streak plate method, 37 degree Celsius incubator
E)sterile swab, streak plate method, 37 degree Celsius incubator

Question

Quizplus · Accepted Answer

To isolate single colonies, the streak plate method should be used with an inoculating loop to streak the mixed culture onto the agar plate, then incubated at 37 degrees Celsius. The incinerator is used to sterilize the loop between streaks to prevent contamination.

In Lab, Tom Was Given a Mixed Culture