Which of the following reagents reacts with crystal violet as the mordant?
A)crystal violet
B)Gram's iodine
C)95% ethyl alcohol
D)safranin

Question

Quizplus · Accepted Answer

Gram's iodine is the reagent that reacts with crystal violet as the mordant in the Gram staining process. This reaction results in the formation of a stable crystal violet-iodine complex in the cell walls of Gram-positive bacteria, which helps to retain the purple stain after decolorization with alcohol. In contrast, Gram-negative bacteria are unable to retain the crystal violet-iodine complex and are subsequently stained by the counterstain, safranin.

Which of the Following Reagents Reacts with Crystal Violet as the Mordant