You are part of a research team trying to sequence the genome of bacterial species X,which has a genome size of 2.2 Mb.You plan to use shotgun DNA sequencing and want to sequence at least 99% of the genome.How many Mb of DNA should you sequence?
A)10.13
B)4.61
C)0.01
D)9.02

Question

Quizplus · Accepted Answer

To ensure 99% coverage of a 2.2 Mb genome with shotgun sequencing, a common approach is to aim for a certain level of redundancy or coverage depth. The Lander-Waterman equation is often used to calculate the necessary sequencing effort. For 99% coverage, the equation suggests that the coverage depth (C) needed is approximately -ln(1-0.99), which is about 4.6. Therefore, to achieve this coverage for a 2.2 Mb genome, you would need 4.6 times the genome size in sequencing, which is 4.6 * 2.2 Mb = 10.12 Mb. Rounded to two decimal places, the answer is 10.13 Mb.

You Are Part of a Research Team Trying to Sequence