Bam HI is a Type II restriction enzyme that recognizes the sequence 5' GGATCC 3',and cuts between the two Gs,leaving a 5' overhang.If you were to digest DNA with Bam HI,what would be the sequence of the overhanging sticky end of the complimentary strand?
A)5' GATCC 3'
B)5' GATC 3'
C)5' CTAG 3'
D)5' G 3'

Question

Quizplus · Accepted Answer

Bam HI cuts between the two G's in the recognition sequence, leaving a 5' overhang on the complementary strand. Therefore, the sequence of the overhanging sticky end of the complementary strand would be 5' GATC 3'.

Bam HI Is a Type II Restriction Enzyme That Recognizes