Ends of DNA fragments produced by asymmetric cleavage with restriction endonucleases;same ends are produced by the same enzyme irrespective of the source of the DNA.
A)sticky ends
B)RFLP
C)PCR
D)plasmids
E)transgenic organisms

Question

Quizplus · Accepted Answer

Asymmetric cleavage with restriction endonucleases produces sticky ends, which are single-stranded overhangs at the ends of DNA fragments. These overhangs can base pair with complementary overhangs on other fragments, allowing for the fragments to be joined together. The same enzyme will always produce the same sticky ends, regardless of the source of the DNA. RFLP is a technique used to detect variations in DNA sequences, PCR is a technique used to amplify DNA, plasmids are small, circular pieces of DNA often used in genetic engineering, and transgenic organisms are organisms that contain foreign DNA that has been artificially introduced. None of these choices specifically relate to the production of sticky ends through asymmetric cleavage with restriction endonucleases.

Ends of DNA Fragments Produced by Asymmetric Cleavage with Restriction