Which of the following is NOT true for HPLC sample injection. A)Reproducibly load injector loop with 3 loop volumes depending on injection technique. B)The injection loops must be removed and cleaned after each injection. C)Sample solvent strength should be less than or equal to eluent solvent strength. D)Pass samples through a 0.5-$\mu$m filter to remove particulate matter prior to injection. E)Use a blunt HPLC syringe needle,not pointed,to prevent damage to the injection port.

Question

Which of the following is NOT true for HPLC sample injection.
A)Reproducibly load injector loop with < 0.5 or > 3 loop volumes depending on injection technique.
B)The injection loops must be removed and cleaned after each injection.
C)Sample solvent strength should be less than or equal to eluent solvent strength.
D)Pass samples through a 0.5-$\mu$m filter to remove particulate matter prior to injection.
E)Use a blunt HPLC syringe needle,not pointed,to prevent damage to the injection port.

Quizplus · Accepted Answer

Injection loops do not necessarily have to be removed and cleaned after each injection, as long as there is no residual material left in the loop that could potentially interfere with subsequent injections. This can be verified by running blank injections or flushing the loop with a suitable solvent between injections.

Which of the Following Is NOT True for HPLC Sample μ\muμ

Which of the Following Is NOT True for HPLC Sample $\mu$