Which of the following are characteristics of reverse-phase chromatography?
I The stationary phase is polar.
II The mobile phase is more polar than the stationary phase.
III Less polar mobile phase has a lower eluent strength.
IV More polar mobile phase has a higher eluent strength.
V The stationary phase is nonpolar.
A)I and IV
B)II only
C)I and III
D)II and IV
E)II and V

Question

Quizplus · Accepted Answer

In reverse-phase chromatography, the stationary phase is nonpolar (V), and the mobile phase is more polar than the stationary phase (II). This setup is opposite to normal-phase chromatography, where the stationary phase is polar.

Which of the Following Are Characteristics of Reverse-Phase Chromatography