The DNA primers used in PCR are
A) identical to the entire base sequence of one strand of the DNA.
B) produced when a gene of interest is read by restriction enzymes.
C) attached to the gene of interest by ligase.
D) complementary to DNA sequences at both ends of the DNA sequence of interest.

Question

Quizplus · Accepted Answer

The DNA primers used in PCR are synthesized to be complementary to the DNA sequences at the 5' and 3' ends of the target DNA sequence. This allows them to anneal to the DNA and provide a starting point for the polymerase to amplify the DNA of interest. A and B are incorrect because the primers do not need to be identical to the entire base sequence of one strand of DNA, nor are they produced by restriction enzymes. C is incorrect because ligase is not involved in attaching primers to the gene of interest in PCR.

The DNA Primers Used in PCR Are