Question 1

Knockout libraries are available already prepared for some organisms such as Drosophila.These libraries are originally made by what method?

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Question 2

In an organism,X,it is found to be impossible to generate loss-of-function mutants for most genes.There is an alternative method of introducing random mutations that can later be screened for the gene(s)in question.This can be accomplished by using insertions due to what structures?

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Question 3

One way to genetically engineer S.cerevisiae is by homologous recombination.If a given sequence is thus introduced and is integrated via two regions of homology,is it more probably introduced as a linear or a circular entity?

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Question 4

If a gene is replaced with a heterologous sequence such that the gene is then nonfunctional,what is the result usually called?

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Question 5

If a mutagen such as EMS is used to produce numerous mutagenized cell lines for an organism,PCR can be used to select for a particular gene.However,what feature of this selected sequence is used to find the ones with mutations in that gene?

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Question 6

In the formation of transgenic animals,DNA is usually inserted into eggs or embryos.If some cells of the embryo do not receive the introduced DNA,the result is an organism with cells of different genotypes;the organism is known as what?

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Question 7

In nature,RNAi protects cells against infection by what kind of pathogen?

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Question 8

If a researcher is cloning a sequence by using pUC and uses the plasmid to transform bacteria,which are then plated on X-gal medium,would the recombinant plasmids be found in blue or white colonies or both?

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Question 9

For a transgenic animal line to be produced from a chimera,the transgene must be carried by what?

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Question 10

What is the function of imaginal disc cells during the larval stage of Drosophila development?

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Question 11

By what method is T-DNA integrated into the plant genome?

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Question 12

The GFP gene is useful as a reporter gene because the cells and tissues with it emit bioluminescence when treated with what,that functions as a substrate?

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Question 13

If an experimenter wants to use the GFP method but needs to detect the presence or absence of several proteins at the same time,he can take advantage of mutational variants of GFP that emit what?

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Question 14

If a plant species cannot readily have a new gene integrated into it via a Ti plasmid,it must then be subjected to different methods.These often include removing the cell wall and inserting DNA,after treating the membrane with heat or electricity to do what to the membrane?

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Question 15

In producing an E.coli plasmid to be an expression vector,an experimenter includes genes for selectable markers,a promoter for the expression of the introduced genes.She uses a cDNA sequence to ensure the insert contains no introns.The gene inserted is transcribed,but not translated.What sequence did she omit from the plasmid?

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Question 16

In grape cultivation,growers often find that heterozygous forms are more desirable.To propagate the heterozygotes without having to interbreed in each generation,what method can they use?

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Question 17

Agrobacterium tumefaciens is often used in the production of transgenic plants because of its Ti plasmid.This plasmid is usually "disarmed" by deleting its tumor genes along with the genes for producing what products that would have been advantageous to the bacterium?

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Question 18

In the pUC plasmids,a polylinker region that includes multiple restriction sites is embedded in the lacZ component.If a sequence of interest is inserted into this region,what is the effect on lacZ?

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Question 19

In the case of Jesse Gelsinger,a young man who died during gene therapy,the therapy involved delivery via a viral vector.Was this germ-line or somatic therapy?

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Question 20

What kind of gene silencing is induced by short double-stranded RNAs?

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Quiz 17: Applications of Recombinant DNA Technology and Reverse Genetics