If a protein to be overexpressed is toxic to the expression host,it is best to select an expression vector that
A) is compatible with a binary vector able to be regulated.
B) is inducible.
C) has a relatively low copy number per cell.
D) prevents folding of the overexpressed protein into its toxic form.

Question

Quizplus · Accepted Answer

Selecting an inducible expression vector allows for better control over when the protein is produced. This means that the toxic effects of the protein can be minimized by inducing expression only when necessary or under controlled conditions. Option C could also be a consideration, as a lower copy number would result in less toxic protein being produced overall. However, this may not be enough to completely prevent toxicity. Option A may not be necessary in this case, as regulating the binary vector would not directly address the toxicity of the expressed protein. Option D is not a realistic option, as preventing folding of the expressed protein would likely compromise its function.

If a Protein to Be Overexpressed Is Toxic to the Expression