The enzyme that covalently links both strands of a vector and inserted DNA in molecular cloning is
A) DNA ligase.
B) DNA phosphatase.
C) DNA hydrolase.
D) DNA transferase.

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme that catalyzes the formation of phosphodiester bonds between the nucleotides of the vector and inserted DNA, covalently linking them together to form a recombinant DNA molecule. DNA phosphatase, DNA hydrolase, and DNA transferase are not involved in this process.

The Enzyme That Covalently Links Both Strands of a Vector