Some proteins expressed at high levels form inclusions that are relatively insoluble. What is the most effective way to facilitate purification of these proteins?
A) Use a reporter gene to locate the inclusion.
B) Decrease the number of insertions in the vector.
C) Produce the protein as a fusion protein.
D) Switch to an expression host with a larger intracellular volume.

Question

Quizplus · Accepted Answer

Producing the protein as a fusion protein with a solubility tag or partner can increase solubility of the protein and facilitate purification.

Some Proteins Expressed at High Levels Form Inclusions That Are