Question 1

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to detect the presence of red cell antibodies and B cells A)Panel cells B)Screening cells C)A₁and B cells D)ABO antisera E)Lectins

Accepted Answer

The answer of Select the reagent from the list below...

Question 2

The purpose of adding antibody-sensitized red cells following the antiglobulin test is to:
A)make sure a weak antibody reaction was not missed.
B)confirm positive reactions.
C)check that the wash procedure was sufficient to remove unbound antibodies.
D)check that sufficient incubation took place.

Accepted Answer

Antibody-sensitized red cells (check cells)are IgG-coated cells that will detect unbound antihuman globulin following proper washing techniques.

Question 3

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to determine the ABO antigenic composition of a patient's red cells A)Panel cells B)Screening cells C)A₁and B cells D)ABO antisera E)Lectins

Accepted Answer

ABO antisera are used to determine the ABO antigenic composition of a patient's red cells by agglutinating with the corresponding antigens on the red cells.

Question 4

What immunoglobulin class reacts best by antiglobulin testing?
A)IgM
B)IgA
C)IgE
D)IgG

Accepted Answer

The antiglobulin test detects IgG antibodies on red cells.

Question 5

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to identify the specificity of a red cell antibody antisera A)Panel cells B)Screening cells C)A₁and B cells D)ABO antisera E)Lectins

Accepted Answer

Panel cells are used to identify the specificity of a red cell antibody antisera. The panel cells are a collection of red blood cells with known antigenic specificities that are used to determine the specificity of an unknown antibody present in the patient's serum.

Question 6

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies A)Panel cells B)Screening cells C)A₁and B cells D)ABO antisera E)Lectins

Accepted Answer

A1 and B cells are used in the identification of ABO antibodies because they can help differentiate between the ABO blood groups by reacting with the antibodies present in the patient's serum.

Question 7

Select the antiglobulin test that best fits the descriptions below.
 selection may be used more than once.
-Requires washing the cells several times before the addition of antihuman globulin reagent
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

The answer of Select the antiglobulin test that best fits...

Question 8

Select the antiglobulin test that best fits the descriptions below.
 selection may be used more than once.
-Detects IgG or complement-coated red cells
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

Both the direct and indirect antiglobulin test can be used to detect IgG or complement-coated red cells. The direct test is used to detect antibodies already attached to the surface of red blood cells in the patient's body, while the indirect test is used to detect antibodies present in the serum against red blood cells. Both can identify the presence of IgG or complement on red cells.

Question 9

Select the antiglobulin test that best fits the descriptions below.
 selection may be used more than once.
-Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

The answer of Select the antiglobulin test that best fits...

Question 10

Rouleaux is a false-positive reaction that would not likely be observed during which of the following tests?
A)Immediate spin antibody screen
B)Weak D test
C)Reverse typing in the ABO test
D)ABO forward typing

Accepted Answer

Rouleaux are caused by an elevated protein level or IV solutions and cause cells to appear agglutinated.A procedure involving washing,such as the direct or indirect antiglobulin test,would not be affected by this because saline would eliminate the excess proteins.

Question 11

Which of the following red cell antigens do proteolytic enzymes destroy? A)Rh system antigens B)Antigens Fy^a and Fy^b in the Duffy system C)Antigens in the Kidd system D)Lewis system antigens

Accepted Answer

Proteolytic enzymes such as ficin will destroy some antigens on red cells such as Fy^a and Fy^b M,N,S.

Question 12

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A₁ from group A₂ red cells A)Panel cells B)Screening cells C)A₁and B cells D)ABO antisera E)Lectins

Accepted Answer

Lectins are reagents derived from plants that are used to distinguish between certain red blood cell groups, such as A₁ and A₂. Lectins are often used in routine blood banking procedures for identifying blood groups and for cross-matching donor and recipient blood. The other options listed (panel cells, screening cells, A₁and B cells, and ABO antisera) are all also used in routine blood banking procedures, but do not specifically match the given description of the reagent derived from plants.

Question 13

Polyethylene glycol is a reagent that can be added to the screen or panel in order to:
A)enhance detection of IgM antibodies.
B)eliminate the reactivity of certain antigen.
C)increase the avidity of IgG antibodies.
D)eliminate the need for washing in the indirect antiglobulin test.

Accepted Answer

Polyethylene glycol (PEG)concentrates antibodies and increases the rate of antibody uptake,increasing the avidity of IgG antibody reactions.

Question 14

Select the antiglobulin test that best fits the descriptions below.
 selection may be used more than once.
-Incubation step is not necessary
A)Indirect antiglobulin test
B)Direct antiglobulin test
C)Both the direct and indirect antiglobulin test

Accepted Answer

The direct antiglobulin test (DAT) does not require an incubation step. The DAT involves the direct addition of antiglobulin reagent to patient cells, usually red blood cells, to detect IgG or complement bound to the cells.

Quiz 2: Blood Banking Reagents: Overview and Applications

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to detect the presence of red cell antibodies and B cells

The purpose of adding antibody-sensitized red cells following the antiglobulin test is to:

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used to determine the ABO antigenic composition of a patient's red cells

What immunoglobulin class reacts best by antiglobulin testing?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent to identify the specificity of a red cell antibody antisera

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent used in the identification of ABO antibodies

Select the antiglobulin test that best fits the descriptions below. selection may be used more than once. -Requires washing the cells several times before the addition of antihuman globulin reagent

Select the antiglobulin test that best fits the descriptions below. selection may be used more than once. -Detects IgG or complement-coated red cells

Select the antiglobulin test that best fits the descriptions below. selection may be used more than once. -Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

Rouleaux is a false-positive reaction that would not likely be observed during which of the following tests?

Which of the following red cell antigens do proteolytic enzymes destroy?

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A1 from group A2 red cells

Polyethylene glycol is a reagent that can be added to the screen or panel in order to:

Select the antiglobulin test that best fits the descriptions below. selection may be used more than once. -Incubation step is not necessary

Select the reagent from the list below and match it to the routine blood banking procedure. -Reagent derived from plants used to distinguish group A₁ from group A₂ red cells