What method is good for separating large DNA fragments in a low-percentage, low-melt agarose gel by an angled electrical field that periodically changes orientation?
A)Restriction fragment length polymorphism (RFLP)
B)Southern blot
C)Agarose gel electrophoresis
D)Pulsed field gel electrophoresis (PFGE)

Question

Quizplus · Accepted Answer

A restriction enzyme is used to digest chromosomal DNA at a small number of sites along the chromosome.This results in large DNA fragments that are difficult to resolve by standard agarose gel electrophoresis.During PFGE, these large fragments are separated in a low-percentage, low-melt agarose gel by an angled electrical field that periodically changes orientation.These changes in electrical field orientation are pulses that successfully "force" the large DNA fragments through the agarose gel and separate them by size.A low-percentage agarose gel is used for the large DNA fragments, and low-melt agarose also enables large fragments to migrate easier.

What Method Is Good for Separating Large DNA Fragments in a Low-Percentage