The goal of the primer annealing step is to
A) hybridize oligonucleotide primers to the denatured, single-stranded target DNA strands.
B) separate the target DNA so that the solution can be electrophoresed and a Northern blot performed.
C) cut the native DNA into small pieces with a restriction enzyme.
D) amplify exponentially over many cycles of these three reaction steps.

Question

Quizplus · Accepted Answer

The primer annealing step's goal is to hybridize oligonucleotide primers to the denatured, single-stranded target DNA strands. This is a crucial step for the subsequent amplification step because the primers provide the starting point for DNA polymerase to extend the complementary strand.

The Goal of the Primer Annealing Step Is to