It is difficult to determine either Km or Vmax from a graph of velocity vs.substrate concentration because
A)too much substrate is required to determine them.
B)the graph is sigmoidal.
C)an asymptotic value must be determined from the graph.
D)the points on the graph are often not spread out on the hyperbola.

Question

Quizplus · Accepted Answer

An asymptotic value must be determined from the graph to accurately estimate Vmax, and Km is derived from the relationship between Vmax and the substrate concentration at which the reaction velocity is half of Vmax. This makes direct determination from a simple velocity vs. substrate concentration graph challenging without further analysis or transformation of the data.

It Is Difficult to Determine Either Km or Vmax from a Graph