In a single PCR cycle consisting of 15 seconds at 94°C,30 seconds at 50°C,and 1 min at 72°C,what is happening in the step run at 50°C?
A)The DNA to be amplified is being denatured.
B)Primers are being denatured.
C)DNA polymerase is extending new DNA from the primers.
D)Primers are annealing to the DNA to be amplified.
E)DNA polymerase is being inactivated.

Question

Quizplus · Accepted Answer

During the step run at 50°C, the primers are annealing to the single-stranded DNA template to be amplified. This step is crucial for the amplification to occur as it provides a starting point for the DNA polymerase to extend new DNA strands.

In a Single PCR Cycle Consisting of 15 Seconds at 94°C,30