The restriction enzymes BamHI and BclI cut at the points indicated by arrows:

If one sample of DNA was cut with BamHI and another with BclI,and these two samples were mixed and treated with DNA ligase,what would occur?
A)No DNAs would be ligated (joined together).
B)Only the BamHI-cut DNA would be ligated.
C)Only the BclI-cut DNA would be ligated.
D)Both BamHI-cut and BclI-cut DNAs would be ligated,but only to DNA fragments cut with the same enzyme (e.g. ,BamHI fragments would be ligated only to other BamHI fragments).
E)Both BamHI-cut and BclI-cut DNAs would be ligated with no preference for which fragment is ligated to which other.

Question

The restriction enzymes BamHI and BclI cut at the points indicated by arrows:

If one sample of DNA was cut with BamHI and another with BclI,and these two samples were mixed and treated with DNA ligase,what would occur?
A)No DNAs would be ligated (joined together). 
B)Only the BamHI-cut DNA would be ligated. 
C)Only the BclI-cut DNA would be ligated. 
D)Both BamHI-cut and BclI-cut DNAs would be ligated,but only to DNA fragments cut with the same enzyme (e.g. ,BamHI fragments would be ligated only to other BamHI fragments). 
E)Both BamHI-cut and BclI-cut DNAs would be ligated with no preference for which fragment is ligated to which other.

Quizplus · Accepted Answer

Both BamHI-cut and BclI-cut DNAs would be ligated with no preference for which fragment is ligated to which other. DNA ligase can join together any two ends of DNA that are complementary, regardless of how the ends were generated. Therefore, both BamHI-cut and BclI-cut DNAs would be ligated with no preference for which fragment is ligated to which other. However, if two different restriction enzymes generate incompatible ends, such as sticky ends produced by BamHI and blunt ends produced by BclI, ligation would not be possible.

The Restriction Enzymes BamHI and BclI Cut at the Points