Positional cloning allows researchers to isolate a gene without having knowledge of its biochemical basis. Linkage studies are used to map the locus producing a phenotype of interest to a particular chromosome region. Chromosome walking and jumping are used to progress from molecular makers to clones containing sequences that cover the chromosome region. Candidate genes within the region are then evaluated to determine if they encode the phenotype of interest.
-How are candidate genes that are identified by positional cloning evaluated to determine whether they encode the phenotype of interest?

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The Answer of Positional cloning allows researchers to isolate a gene without having knowledge of its biochemical basis. Linkage studies are used to map the locus producing a phenotype of interest to a particular chromosome region. Chromosome walking and jumping are used to progress from molecular makers to clones containing sequences that cover the chromosome region. Candidate genes within the region are then evaluated to determine if they encode the phenotype of interest.
-How are candidate genes that are identified by positional cloning evaluated to determine whether they encode the phenotype of interest?

Positional Cloning Allows Researchers to Isolate a Gene Without Having