The polymerase chain reaction is an enzymatic in vitro method for rapidly amplifying DNA. In this process, DNA is heated to separate the two strands, short primers attach to the target DNA, and DNA polymerase synthesizes new DNA strands from the primers. Each cycle of PCR doubles the amount of DNA. PCR has a number of important applications in molecular biology.
-Why is the use of a heat-stable DNA polymerase important to the success of PCR?
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