The final step in a DNA sequencing reaction is to run the DNA fragments on a gel.What purpose does this serve?
A)It provides the substrate for DNA polymerase.
B)It separates dideoxynucleotides from deoxynucleotides.
C)It terminates the sequencing reaction at particular bases.
D)It separates DNA fragments generated during the sequencing reaction based on one-nucleotide differences in their size.

Question

Quizplus · Accepted Answer

Running the DNA fragments on a gel separates them based on their size, specifically one-nucleotide differences in size. This allows the identification of the specific bases present in the DNA sequence being sequenced.

The Final Step in a DNA Sequencing Reaction Is to Run