Question 1

You have been exposed to Franciscella tularensis due to a faulty laminar flow hood. Your supervisor knew the hood was not functioning properly but told you to continue using it because it was better than nothing. To report this violation, you should notify

Accepted Answer

OSHA (Occupational Safety and Health Administration) is responsible for ensuring safe and healthy working conditions by setting and enforcing standards, and by providing training, outreach, education, and assistance. Reporting a violation related to workplace safety, such as being exposed to a hazardous biological agent due to inadequate equipment, falls under OSHA's jurisdiction.

Question 2

The NFPA label is used to identify _____ hazards.

Accepted Answer

The NFPA label is specifically designed to identify chemical hazards, as part of the National Fire Protection Association's system for informing about the health, flammability, reactivity, and special hazards of chemicals.

Question 3

A substance or microorganism that can cause infection, allergy, or toxicity is known as

Accepted Answer

A biohazard is a substance or microorganism that poses a threat to human health, including those that can cause infection, allergy, or toxicity.

Question 4

You are processing a cerebrospinal fluid for culture for Neisseria meningitidis. To protect yourself and others, you will need to use
1) a biological safety cabinet.
2) gloves.
3) a fastened lab coat.
4) safety glasses.

Accepted Answer

The answer of You are processing a cerebrospinal fluid for...

Question 5

A biological safety cabinet functions by

Accepted Answer

A biological safety cabinet is designed to provide protection in three main ways: it acts as a barrier between the specimen and the microbiologist (A), maintains negative pressure to prevent the escape of aerosols into the laboratory environment (B), and protects samples from external contamination (C). Therefore, all of the statements are correct.

Question 6

You have accepted an evening position in a hospital microbiology department. Your new employer should provide you with

Accepted Answer

The answer of You have accepted an evening position in...

Question 7

Isolation rooms and the use of HEPA filters and ultraviolet light are considered

Accepted Answer

Isolation rooms, HEPA filters, and ultraviolet light are considered engineering controls because they involve the use of physical or mechanical means to reduce the spread of infectious agents by controlling the environment.

Question 8

In order to work with cultures of Mycobacterium tuberculosis, the laboratory must be categorized as

Accepted Answer

Mycobacterium tuberculosis requires Biosafety Level 3 (BSL 3) containment due to its airborne transmission risk and the serious disease (tuberculosis) it causes. BSL 3 labs have specific engineering and procedural controls to protect workers and the environment.

Question 9

A medical center's microbiology laboratory has locked keyless entry doors that require a number code to enter. This laboratory is probably a BSL Level

Accepted Answer

BSL-3 (Biosafety Level 3) laboratories have special engineering and design features to prevent microorganisms from being released into the environment, including controlled access zones such as keyless entry doors requiring a numeric code. These labs work with pathogens that can cause serious and potentially lethal diseases via inhalation.

Question 10

The isolation of ___________________ from a patient sample should activate the microbiology department's plan for isolation of potential bioterrorism agents.

Accepted Answer

Yersinia pestis is the causative agent of the plague and is considered a potential bioterrorism agent due to its historical significance and potential for high mortality if used in an aerosolized form. The isolation of Yersinia pestis from a patient sample would necessitate immediate action according to protocols for handling potential bioterrorism agents.

Question 11

A small Midwest hospital has isolated what is strongly suspected to be Bacillus anthracis from three different individuals who are postal employees. The department supervisor should

Accepted Answer

The answer of A small Midwest hospital has isolated what...

Question 12

You have finished a run of samples for detection of Neisseria gonorrhoeae by nucleic acid amplification. The kit controls did not work. This is an example of a/an ______________ error(s) in the laboratory testing process.

Accepted Answer

Analytical errors occur during the testing process itself, which includes issues with the test kit or equipment malfunctions, such as controls not working properly.

Question 13

Using a 10% suspension of bleach to clean a work bench is considered

Accepted Answer

Using a 10% suspension of bleach to clean a workbench is considered disinfection because it eliminates many or all pathogenic microorganisms on inanimate objects, except bacterial spores. Sterilization, on the other hand, would eliminate all forms of microbial life, including spores.

Question 14

Alcohol is not used to clean the work surfaces in a microbiology department because it

Accepted Answer

Alcohol is not effective against bacterial spores, which are resistant to many disinfectants, including alcohol. This makes it unsuitable for cleaning work surfaces in a microbiology department where sterilization and the elimination of all microbial life forms, including spores, are crucial.

Question 15

Standard precautions are intended to protect

Accepted Answer

Standard precautions are designed to protect health care workers, patients, and visitors by minimizing the risk of infections spreading.

Question 16

When working in the microbiology laboratory, standard precautions involve the use of gloves

Accepted Answer

Standard precautions require the use of gloves when there is a potential for contact with body fluids to prevent exposure to infectious agents.

Question 17

The single most important technique used to prevent the spread of nosocomial infection is

Accepted Answer

Hand washing is widely recognized as the single most important practice in preventing the spread of nosocomial (hospital-acquired) infections. It removes or destroys pathogens on the hands, significantly reducing the risk of transmitting infections to patients or healthcare workers.

Question 18

Containers for disposal of potentially infectious waste must be
1) puncture proof.
2) sealable.
3) leakproof.
4) specifically labeled using color.

Accepted Answer

The answer of Containers for disposal of potentially infectious waste...

Question 19

Proficiency testing evaluates all of the following except

Accepted Answer

The answer of Proficiency testing evaluates all of the following...

Question 20

Which monitor is not usually associated with a microbiology laboratory's quality assurance program?

Accepted Answer

Certification examination pass rate of microbiology personnel is not typically associated with a microbiology laboratory's quality assurance program, as it relates more to staff qualifications and training rather than the direct quality control of laboratory processes and outcomes.

Quiz 5: Safety