Question 1

Earl understands that exposure to body fluids, tissue samples, and cells for processing, entails the observance of which of the following biosafety levels?
A)Biosafety Level 1
B)Biosafety Level 2
C)Biosafety Level 3
D)Biosafety Level 4

Accepted Answer

Biosafety Level 2
Handling and exposure to potentially infectious biologic entities such as extracted body fluids, samples of tissues, and cells used for processing, requires the laboratory personnel to observe biosafety level 2. This biosafety level includes standard microbiological practices and the placement of biohazard signs on areas that receive and process these samples that are potentially infected with human diseases such as hepatitis, influenza, and Staphylococcus infection. Other choices do not reflect the required biosafety level for handling and processing tissue samples, body fluids, and cells from a patient.

Question 2

Frank remembers that the correct set of personal protective equipment required for biosafety level 1 is which of the following:
A)Sterile gloves, lab gown, eye patch, and proper footwear
B)Working gloves, lab coat, goggles, and wooden sandals
C)Gloves, lab gown, eye protection, and appropriate footwear
D)Gloves, lab coat, eyeglasses, and proper footwear

Accepted Answer

Gloves, lab gown, eye protection, and appropriate footwear
The recommended set of personal protective equipment that is needed to observe biosafety level 1 is composed of: a pair of latex or nitrile gloves, a lab coat or a lab gown, eye protection, specifically a pair of googles, and appropriate footwear such as a closed shoes and closed sandals are deemed adequate. Other choices are not the correct set as these contain equipment that does not meet the standards.

Question 3

Gerald knows that among the following equipment, one of which protects the worker, the products stored and the environment.
A)Chemical fume hoods
B)Biosafety cabinets
C)Clean benches
D)Refrigerator

Accepted Answer

biosafety cabinets
The specific equipment that protects the health care workers, the products being stored, and the environment is the biosafety cabinet. Chemical fume hoods only protect the health care worker while clean benches only protect the products being stored. The refrigerator is not a biosafety equipment.

Question 4

Houston is correct when he identifies which among the following practices are correct in wearing gloves as a PPE, except:
A)Gloves are donned when handling biohazardous materials.
B)Nitrile gloves must be worn when processing materials of extreme temperatures.
C)Gloves must always overlap the lab coat sleeves to ensure protection.
D)The practice of "double gloving" provides more protection in some situations.

Accepted Answer

Nitrile gloves must be worn when processing materials of extreme temperatures
The following choices are true and correct about donning gloves as a form of personal protective equipment in the laboratory, except for using nitrile gloves when processing materials of extreme temperatures. This is because nitrile gloves cannot withstand extremes in temperatures. Thus, temperature-resistant gloves are used for this purpose especially when handling hot objects or dry ice.

Question 5

Ice is correct when he practices which of the following principles when dealing with sharps in the laboratory?
A)Request removal of the sharps container when it is already almost full.
B)Needles can be recapped with the fingers of the other hand using extra precaution.
C)Always utilize a mechanical tool when removing knives, and other types of sharps.
D)Broken glassware can be handled directly using a double-gloved hands.

Accepted Answer

Needles can be recapped with the fingers of the other hand using extra precaution.
The correct practice when dealing with sharps is to always utilize a mechanical tool when removing knives, blades, and other types of sharps. The sharps container must be replaced when it is already about 2/3 full. Fingers are not recommended when removing or recapping needles even with extra precaution. Broken glassware should not be handled manually even on double-gloved hands.

Question 6

Jay is correct when he adheres to the following recommended practices for handling human cells and tissues in the clinical laboratory
A)All work with such cells and tissues should be done in a biosafety cabinet.
B)All potentially infected samples should be decontaminated by autoclaving only.
C)All laboratory staff is not required to submit blood samples for reference.
D)All laboratory personnel should strictly follow the BSL-1 recommendation.

Accepted Answer

All work with such cells and tissues should be done in a biosafety cabinet.
It is recommended that when handling human cells and tissues in the laboratory, all work with such specimens should be done in a biosafety cabinet. All potentially infected samples can be decontaminated by way of autoclaving or by disinfection. It is also recommended that all laboratory staff must submit blood samples to serve as a baseline data for reference. All laboratory personnel should strictly follow the BSL-2 recommendation when handling these types of specimens.

Question 7

Dexter understands that the following principles of biosafety are correct, except:
A)The primary goal of biosafety is to contain potentially infectious biological materials.
B)Containing potentially infectious agents is aimed at reducing exposure to these hazardous materials.
C)Utilization of vaccines on health care workers will surely protect against harmful pathogens.
D)Doing assessment of risks when handling potentially hazardous materials can be generalized with a group of specimens to increase efficiency.

Accepted Answer

Doing assessment of risks when handling potentially hazardous materials can be generalized with a group of specimens to increase efficiency.
All the other principles of biosafety are correct except for doing an assessment of risks when handling potentially hazardous materials can be generalized with a group of specimens to increase efficiency. This is because the assessment of risks should be ideally done by every specific agent since it may entail special precautions and considerations and must not be generalized.

Question 8

Joy knows that observation of the following laboratory practices is correct.
A)Containment is effective when standard microbiological procedures are often observed and followed.
B)Personnel working with potentially infectious agents should be aware of the potential hazards of handling such specimens.
C)Training on proper handling of potentially infected samples can be substituted by gaining experience in the laboratory.
D)The laboratory personnel are responsible for self training on such essential laboratory techniques and principles.

Accepted Answer

Personnel working with potentially infectious agents should be aware of the potential hazards of handling such specimens.
One correct observation of laboratory practices is for the laboratory personnel to be aware of the potential hazards of handling specimens. Containment is only effective when standard microbiological procedures are always strictly followed. Training on proper handling of potentially infected samples can never be substituted by experience and so training is a must. The director of the laboratory is responsible for coordinating the training needs of his or her laboratory personnel.

Question 9

Mark is correct to identify which among the following is not a form of safety equipment in the laboratory?
A)Biosafety cabinet
B)Enclosed containers
C)Fume hood
D)Engineering controls

Accepted Answer

Engineering controls
The following choices are examples of safety equipment in the laboratory except for engineering controls. Although engineering controls covers a wide range of technology to limit exposure, contain potentially hazardous materials, and serve as barriers, the concept itself is not a form of safety equipment in the laboratory.

Question 10

Noel knows that which among the following statements is true regarding biosafety cabinets.
A)There are four classes of biosafety cabinets used in the laboratories.
B)Both Class I and Class II biosafety cabinets are able to serve as primary barriers that protects both the lab workers and the environment.
C)Class II biosafety cabinets are not able to provide protection from external contamination of materials.
D)Class IV biosafety cabinets provide the greatest level of protection for both the lab personnel and the environment.

Accepted Answer

Among the choices above, the statement that both Class I and Class II biosafety cabinets are able to serve as primary barriers that protects both the lab workers and the environment is true regarding biosafety cabinets. In the laboratory, there are only three classes of biosafety cabinets. Class II biosafety cabinets are also able to provide protection from external contamination. Class III biosafety cabinets provide the greatest level of protection.

Question 11

Orlando knows that the following are primary barriers used inside the laboratory, except:
A)Safety centrifuge cup
B)Shoe covers
C)Reading glasses
D)Face shield

Accepted Answer

All the following choices mentioned above are primary barriers used inside the laboratory except for reading glasses. Standard eye protection that is accepted are goggles and safety glasses. Sunglasses and reading glasses would not provide adequate eye protection inside the laboratory.

Question 12

Paul remembers correctly that which of the following is not a secondary barrier employed in the laboratory?
A)Biosafety cabinet
B)Division of the specific areas in the laboratory
C)Decontamination facility
D)Autoclave machine

Accepted Answer

Biosafety cabinet
All the following options are concrete examples of secondary barriers employed in clinical laboratories except for the biosafety cabinet. A lab's secondary barrier is comprised mainly of the unique design of the facility to separate the laboratory from the common access of the public. A lab should also contain a decontamination facility such as the use of an autoclave.

Question 13

Quark is correct to identify which among the biosafety levels are applied when working with samples that might contain the following microorganisms: Bacillus subtilis, Nigeria gruberi, and infectious canine hepatitis virus?
A)BSL - 2
B)BSL - 3
C)BSL - 1
D)BSL - 4

Accepted Answer

BSL - 1
When handling of specimens is done in the laboratory that might contain the following microorganisms such as Bacillus subtilis, Nigeria gruberi, and infectious canine hepatitis virus, the appropriate biosafety level to be applied is the BSL - 1. This biosafety level 1 is applied when work is done with these microorganisms that are not identified to cause human diseases. All the other biosafety levels in the choices do not accurately address these microorganisms.

Question 14

Roger understands that when dealing with lung tissue that may be infected with Mycobacterium tuberculosis, he must apply which of the following biosafety levels to adequately protect himself and the environment?
A)BSL - 4
B)BSL - 3
C)BSL - 2
D)BSL - 1

Accepted Answer

BSL - 3
When dealing with specimens that might be infected with Mycobacterium tuberculosis, such as in the case of lung tissues, biosafety level 3 must be applied appropriately to ensure effective containment of the pathogen and avoid widespread contamination inside the laboratory. All the other biosafety levels address other pathogens depending on their virulence and potential for widespread contamination.

Question 15

Sylvester is correct when he replies that the following biosafety level is applicable inside the clinical laboratory when working with specimens that are potentially contaminated with pathogens that presents a moderate risk of causing human diseases such as the hepatitis B virus, HIV, and Salmonella species?
A)BSL - 1
B)BSL - 2
C)BSL - 3
D)BSL - 4

Accepted Answer

BSL - 2
BSL - 2 is the biosafety level that is applicable inside the clinical laboratory when dealing with samples that are potentially contaminated with pathogens that presents a moderate risk of causing human diseases. These pathogens include Hepatitis B virus, HIV, Salmonella species and Toxoplasma. This biosafety level is adequate when working with human blood samples, body fluids, tissues, or cells where these pathogens may be present or their presence is unknown. Other choices do not appropriately address the specific pathogens in the question stated above.

Question 16

Tony understands that sterilization in the clinical laboratory can be achieved by the following methods, except:
A)Heating
B)Radiation
C)Ethylene oxide gas
D)Plasma

Accepted Answer

Radiation
Sterilization in the clinical laboratory can be achieved by all of the following methods except for radiation. Although all four methods are used for sterilization, radiation is specifically used for industries and not conducted in clinical laboratories.

Question 17

Uranus is correct when he states that which among the following is correct about the factors that affect the effectiveness of disinfection?
A)The number of non-pathogenic microorganisms
B)The volume of organic matter that is present during disinfection
C)The model or make of the materials to be disinfected
D)The humidity of the environment

Accepted Answer

The volume of organic matter that is present during disinfection
Among the following factors that affect the effectiveness of disinfection, the volume of organic matter that is present during the process of disinfection is correct. The number of pathogenic microorganisms is another factor. The type and condition of the materials to be disinfected matter but not the model or make of such. Lastly, the temperature also affects the effectiveness of disinfection and not the humidity of the environment.

Question 18

Voltaire is correct to identify that instruments that come in contact with sterile parts of the body must require sterilization and is classified under which of the following category?
A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Accepted Answer

Critical Items
According to the Spaulding's classification of instruments based on their risk for infection after possible contamination and their need for sterilization, those instruments that come into contact with sterile parts of the human body that must require sterilization are classified under critical items. All the other choices do not accurately describe the instrument being described in the question stated above.

Question 19

William is right when he classifies items used in the laboratory that constantly comes into contact with the skin. These items can be disinfected with a low to intermediate level of disinfectant as well as by the use of soap and water. In which of the following are these types of items classified?
A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Accepted Answer

Non critical items
Items used in the laboratory that constantly come into contact with our skin is usually disinfected or is cleaned using simple soap and water. These items belong to non critical items according to Spaulding's classification. All the other choices do not accurately describe the type of item being described in the question stated above.

Question 20

Xerxes understands correctly if he categorizes the instruments used inside the clinical laboratory that would require either sterilization or disinfection into which of the following classification?
A)Non critical items
B)Semi-critical items
C)Critical items
D)Extremely critical items

Accepted Answer

Semi critical items
Items or instruments used inside the clinical laboratory that would require either sterilization or disinfection are usually those instruments that come in contact with a patient's cavity or mucous membranes. Therefore, sterilization is not mandatory but can be still be performed. These types of items belong to the semi-critical items on Spaulding's classification.

Quiz 2: Laboratory Operations

Earl understands that exposure to body fluids, tissue samples, and cells for processing, entails the observance of which of the following biosafety levels?

Frank remembers that the correct set of personal protective equipment required for biosafety level 1 is which of the following:

Gerald knows that among the following equipment, one of which protects the worker, the products stored and the environment.

Houston is correct when he identifies which among the following practices are correct in wearing gloves as a PPE, except:

Ice is correct when he practices which of the following principles when dealing with sharps in the laboratory?

Jay is correct when he adheres to the following recommended practices for handling human cells and tissues in the clinical laboratory

Dexter understands that the following principles of biosafety are correct, except:

Joy knows that observation of the following laboratory practices is correct.

Mark is correct to identify which among the following is not a form of safety equipment in the laboratory?

Noel knows that which among the following statements is true regarding biosafety cabinets.

Orlando knows that the following are primary barriers used inside the laboratory, except:

Paul remembers correctly that which of the following is not a secondary barrier employed in the laboratory?

Quark is correct to identify which among the biosafety levels are applied when working with samples that might contain the following microorganisms: Bacillus subtilis, Nigeria gruberi, and infectious canine hepatitis virus?

Roger understands that when dealing with lung tissue that may be infected with Mycobacterium tuberculosis, he must apply which of the following biosafety levels to adequately protect himself and the environment?

Tony understands that sterilization in the clinical laboratory can be achieved by the following methods, except:

Uranus is correct when he states that which among the following is correct about the factors that affect the effectiveness of disinfection?

Voltaire is correct to identify that instruments that come in contact with sterile parts of the body must require sterilization and is classified under which of the following category?

William is right when he classifies items used in the laboratory that constantly comes into contact with the skin. These items can be disinfected with a low to intermediate level of disinfectant as well as by the use of soap and water. In which of the following are these types of items classified?

Xerxes understands correctly if he categorizes the instruments used inside the clinical laboratory that would require either sterilization or disinfection into which of the following classification?