Which of the following is a high-resolution light microscope technique for detecting interactions between proteins?
A) FRAP (fluorescence recovery after photobleaching)
B) FRET (fluorescence resonance energy transfer)
C) MPEM (multi-photon excitation microscopy)
D) Confocal microscopy

Question

Quizplus · Accepted Answer

FRET (fluorescence resonance energy transfer) is a high-resolution light microscope technique for detecting interactions between proteins. It works by measuring the transfer of energy between two fluorescently labeled proteins in close proximity. The energy transfer only occurs if the proteins are interacting, allowing for the detection of protein-protein interactions in living cells.

Which of the Following Is a High-Resolution Light Microscope Technique