In fluorescence microscopy, the immediate source of the light detected is light that has been _______ the sample.
A) absorbed by
B) emitted by
C) exciting
D) scattered from

Question

Quizplus · Accepted Answer

In fluorescence microscopy, a fluorescent molecule in the sample is excited by light of a specific wavelength and then emits light of a longer wavelength, which is the light that is detected. Therefore, the immediate source of the detected light is light that has been emitted by the sample.

In Fluorescence Microscopy, the Immediate Source of the Light Detected