In Which Situation Would Gene Cloning Occur

In which situation would gene cloning occur?

A) A DNA fragment is inserted directly into a bacterium and is replicated.
B) A DNA fragment is inserted into a plasmid, which is then introduced into a bacterium where the gene is expressed, but the plasmid does not replicate.
C) A DNA fragment is inserted into a plasmid, which is then introduced to a bacterium where the plasmid replicates.
D) A DNA fragment is inserted directly into a bacterium and is digested by nucleases.
E) A protein of interest is taken up by a bacterium, which incorporates the protein into its cell membrane.