A researcher wants to insert a DNA fragment into a plasmid. Which enzyme will join the two DNA molecules together?
A) primase
B) β-galactosidase
C) restriction endonuclease
D) DNA ligase
E) DNA polymerase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme responsible for joining two DNA molecules together by forming phosphodiester bonds, making it essential for DNA insertion into plasmids in molecular cloning.

A Researcher Wants to Insert a DNA Fragment into a Plasmid