Multi-cloning sites ("poly-linkers") in most commercially available plasmids offer all the following advantages over the earlier plasmids used in genetic recombination except :
A) They can be used with a wide variety of restriction enzymes.
B) They assure that only the desired DNA will be inserted into the plasmid.
C) They enable easy control of the direction of insertion of the gene of interest.
D) They are usually adjacent to any necessary promoters for gene expression.
E) All of these are advantages.

Question

Quizplus · Accepted Answer

Multi-cloning sites (MCS) or poly-linkers in plasmids are designed to be compatible with many restriction enzymes (A), allow for directional cloning (C), and are often strategically placed near promoters for gene expression (D). However, they do not assure that only the desired DNA will be inserted into the plasmid (B); this depends on the specificity of the restriction enzymes used and the ligation conditions, not the presence of a multi-cloning site itself.

Multi-Cloning Sites ("Poly-Linkers") in Most Commercially Available Plasmids Offer All