The following steps are all involved in genetic recombination:
1) Screening for cells that contain the recombined gene.
2) Cutting the vector with restriction enzyme.
3) Mixing the gene of interest with the vector.
4) Isolating the gene of interest from its original source.
5) Ligating the gene of interest and the vector together.
The following sequence of these five steps would be typical:
A) 1 → 2 → 3 → 4 → 5
B) 2 → 3 → 5 → 1 → 4
C) 5 → 4 → 3 → 2 → 1
D) 4 → 2 → 3 → 5 → 1
E) 2 → 3 → 5 → 4 → 1

Question

Quizplus · Accepted Answer

The correct sequence for genetic recombination involves first isolating the gene of interest (4), then cutting the vector with a restriction enzyme to prepare it for receiving the gene (2), followed by mixing the gene of interest with the vector to allow them to combine (3), ligating the gene of interest and the vector together to create the recombinant DNA (5), and finally, screening for cells that contain the recombined gene to identify successful recombination events (1).

The Following Steps Are All Involved in Genetic Recombination