Indicate whether you would use a fluorescent organic molecule (O), in situ hybridization (H), or a coupled fluorescent protein (P) to visualize the cells and their molecules in each of the following cases. Your answer would be a five-letter string composed of letters O, H, and P only, e.g. OHOOO.
( ) You would like to see where in the early Drosophila embryo the mRNA encoding a certain transcription regulator is located.
( ) You would like to see the nuclei and count them in an early mouse embryo.
( ) You would like to visualize chromosome 3 in a human cell culture derived from a patient's tissue, based on specific sequences present on this chromosome.
( ) You would like to observe the oscillations in Ca²? ions inside a fertilized frog egg.
( ) You would like to compare the localization of two transcription regulatory proteins in cultured human T cells.

Question

Indicate whether you would use a fluorescent organic molecule (O), in situ hybridization (H), or a coupled fluorescent protein (P) to visualize the cells and their molecules in each of the following cases. Your answer would be a five-letter string composed of letters O, H, and P only, e.g. OHOOO.
( )	You would like to see where in the early Drosophila embryo the mRNA encoding a certain transcription regulator is located.
( )	You would like to see the nuclei and count them in an early mouse embryo.
( )	You would like to visualize chromosome 3 in a human cell culture derived from a patient's tissue, based on specific sequences present on this chromosome.
( )	You would like to observe the oscillations in Ca²? ions inside a fertilized frog egg.
( )	You would like to compare the localization of two transcription regulatory proteins in cultured human T cells.

Quizplus · Accepted Answer

Sequence-specific staining of nucleic acids can be achieved by in situ hybridization of probes that are attached to fluorescent dyes. If general DNA staining is desired, a fluorescent organic molecule such as DAPI can be used. Fluorescent or luminescent ion-sensitive indicators (many of which are small molecules) can be used to measure cellular ionic concentrations in real time. A protein can be genetically engineered as a fluorescent fusion protein, and then imaged in living cells by fluorescence microscopy.

Indicate Whether You Would Use a Fluorescent Organic Molecule (O)